This process, occurring within the thylakoid membranes of chloroplasts, represents an alternative electron pathway during the light-dependent reactions. Instead of electrons moving linearly from water to NADPH, they cycle from photosystem I (PSI) back to the cytochrome b6f complex. This, in turn, contributes to the proton gradient across the thylakoid membrane, driving ATP synthesis via chemiosmosis. The primary output of this pathway is ATP; importantly, no NADPH or oxygen is produced in this isolated circuit.
A critical function is to balance the ATP to NADPH ratio within the chloroplast. When NADPH levels are high and the Calvin cycle’s demand for ATP increases, this pathway becomes more prevalent. This ensures sufficient ATP production for carbon fixation. Furthermore, it may offer photoprotective benefits under conditions of high light intensity, preventing photoinhibition by diverting electron flow away from NADPH production. Research suggests its evolutionary significance may lie in its presence in early photosynthetic organisms facing different environmental stressors.